Piperazine-d8 2HCl | CAS 849482-21-9

OVERVIEW  of  Piperazine-d8 2HCl | CAS 849482-21-9

Piperazine-d8·2HCl (CAS 849482-21-9) is a fully deuterated form of piperazine, supplied as its dihydrochloride salt. All eight hydrogen atoms on the two –CH₂– groups of the piperazine ring are replaced by deuterium, providing a stable isotopically labeled analogue of the non-deuterated compound. This reagent is primarily used in NMR spectroscopy, mass spectrometry (MS), and pharmaceutical research as an internal standard, isotopic tracer, or labeled intermediate. The dihydrochloride salt form enhances stability, water solubility, and ease of handling for analytical and synthetic applications.

CHEMICAL INFORMATION

• Name: Piperazine-d8·2HCl

• Molecular Formula: C₄D₈N₂·2HCl

• Molecular Weight: 167.10 g/mol

• CAS Number: 849482-21-9

• Isotopic Labeling: Eight deuterium atoms (fully deuterated ring)

• Chemical Class: Deuterated heterocyclic diamine salt

• Stability: Hygroscopic; stable under recommended storage conditions

APPLICATIONS  of  Piperazine-d8 2HCl | CAS 849482-21-9

• Analytical Chemistry:
  Used as a deuterium-labeled internal standard in LC–MS and GC–MS quantification of piperazine derivatives and related compounds.

• NMR Spectroscopy:
  Provides background signal suppression and serves as a reference compound for chemical shift calibration in studies involving nitrogen-containing heterocycles.

• Pharmaceutical and Medicinal Chemistry:
  Serves as an isotopically labeled intermediate in the synthesis of deuterated analogues of drug candidates containing piperazine moieties.

• Metabolic and Kinetic Studies:
  Used to trace metabolic fate and reaction mechanisms in biological or chemical systems due to its stable isotopic incorporation.

• Isotopic Labeling Research:
  Employed in designing mass-labeled calibration standards and reaction pathway elucidation in advanced analytical workflows.

ADVANTAGES  of  Piperazine-d8 2HCl | CAS 849482-21-9

• High isotopic enrichment (≥ 98 atom % D) for consistent analytical performance.

• Enhanced thermal and chemical stability as the dihydrochloride salt.

• Excellent solubility in polar solvents, particularly water.

• Ideal for high-sensitivity MS and quantitative NMR applications.

• Non-radioactive and environmentally safe isotope label.

HANDLING, STORAGE & SAFETY

• Handling: Handle under dry conditions; avoid moisture exposure.

• Storage: Keep tightly closed in a cool (2–8 °C), dry environment.

• Safety: Low toxicity; may cause mild irritation. Use gloves and eye protection.

• Disposal: Dispose of according to local chemical waste regulations.

QUALITY & SPECIFICATION

• Chemical Purity: ≥ 98 %

• Isotopic Enrichment: ≥ 98 atom % D

• Analytical Validation: Confirmed by ¹H NMR (signal suppression), ²H NMR, IR, and LC–MS

• Documentation: Certificate of Analysis (CoA) available with batch details

SUMMARY
Piperazine-d8·2HCl (CAS 849482-21-9) is a high-purity, fully deuterated cyclic diamine that provides exceptional reliability for quantitative MS, NMR, and labeling research. Its dihydrochloride form ensures chemical stability and solubility, while its complete deuterium substitution makes it ideal for tracer studies, isotopic standardization, and pharmaceutical synthesis. This compound is an indispensable tool in advanced analytical and isotopic chemistry applications.