N-Nitrosomorpholine-d8 | CAS 1219805-76-1

OVERVIEW
N-Nitrosomorpholine-d₈ is the fully deuterated isotopologue of N-nitrosomorpholine (NMOR), a cyclic nitrosamine compound often used as a reference or internal standard in environmental, toxicological, and analytical studies. In this deuterated form, all eight hydrogen atoms of the morpholine ring are replaced with deuterium, resulting in a molecular mass increase of +8 Da while retaining identical chemical and physical behavior to the non-deuterated parent compound.

Due to its stability, isotopic purity, and spectral distinction, N-Nitrosomorpholine-d₈ is widely utilized in gas chromatography–mass spectrometry (GC-MS) and liquid chromatography–mass spectrometry (LC-MS) methods for the quantitative analysis of nitrosamines. It is also valuable for studying metabolic pathways, reaction kinetics, and degradation mechanisms of nitrosamine contaminants in biological and environmental matrices.

CHEMICAL INFORMATION

• Name: N-Nitrosomorpholine-d₈;

• Molecular Formula: C₄D₈N₂O₂

• Molecular Weight: 124.17 g/mol

• CAS Number: 1219805-76-1

• Isotopic Enrichment: ≥ 98 atom % D

• Chemical Class: Deuterated nitrosamine; heterocyclic compound

• Stability: Stable under normal temperature and pressure; decomposes slowly in strong light or under acidic/basic conditions

APPLICATIONS  of  N-Nitrosomorpholine-d8 | CAS 1219805-76-1

• Analytical Chemistry (GC-MS / LC-MS Internal Standard):
  N-Nitrosomorpholine-d₈ is extensively used as an internal standard for the accurate quantification of N-nitrosomorpholine and related nitrosamines in water, food, air, and biological samples. Its isotopic shift allows clear mass spectral separation while maintaining identical chromatographic behavior, ensuring high analytical precision.

• Environmental Monitoring:
  Applied in environmental testing laboratories for detecting nitrosamine pollutants in drinking water, industrial effluents, and atmospheric samples. The deuterated form enhances trace-level quantification reliability and method validation.

• Toxicological and Metabolic Studies:
  Serves as a stable isotope-labeled tracer for studying the metabolism, biotransformation, and carcinogenic potential of nitrosamines in biological systems. It enables researchers to track degradation and metabolic pathways without interference from endogenous compounds.

• Stable Isotope Tracing and Reaction Mechanism Analysis:
  Utilized in mechanistic studies involving nitrosation reactions, oxidative degradation, and photolysis of nitrosamines to understand their environmental persistence and toxicological impact.

• Calibration Standard:
  Functions as a precise calibration reference in mass spectrometric methods to verify instrument performance and analytical consistency in nitrosamine detection assays.

ADVANTAGES  of  N-Nitrosomorpholine-d8 | CAS 1219805-76-1

• High isotopic enrichment (≥98 atom % D) ensures reliable isotopic distinction (+8 Da shift).

• Chemically identical to native N-nitrosomorpholine for direct comparison in analytical assays.

• Excellent stability and reproducibility for long-term method validation.

• Compatible with GC-MS, LC-MS, and NMR spectroscopic applications.

• Non-radioactive, safe isotopic standard for routine laboratory use.

HANDLING

• Hazards: Classified as a potential carcinogen; harmful if swallowed, inhaled, or absorbed through skin. Handle with extreme care and avoid all forms of direct contact.

• Precautions: Work only in a certified fume hood. Wear gloves, protective eyewear, and laboratory coats. Avoid exposure to heat, light, or moisture.

• Storage: Keep tightly closed in amber vials at 2–8 °C. Avoid prolonged exposure to air or light to prevent degradation.

• Disposal: Dispose of waste and contaminated materials according to local hazardous waste regulations for carcinogenic substances.

Safety Note: Although deuterated, N-Nitrosomorpholine-d₈ retains the toxic and potentially carcinogenic properties of its non-deuterated analog. It should therefore be handled using appropriate safety protocols.

QUALITY & SPECIFICATION
Each production batch of N-Nitrosomorpholine-d₈ is subjected to rigorous quality testing to confirm purity, isotopic enrichment, and chemical identity.

• Chemical Purity: ≥ 98 %

• Isotopic Enrichment: ≥ 98 atom % D

• Water Content: ≤ 0.05 %

• Analytical Verification: Validated by ¹H NMR, ²H NMR, IR spectroscopy, and high-resolution mass spectrometry (HRMS)

• COA Provided: Certificate of Analysis available for each lot confirming isotopic composition, purity, and compliance with analytical standards.

SUMMARY
N-Nitrosomorpholine-d₈ (CAS 1219805-76-1) is a highly deuterated isotopic analog of N-nitrosomorpholine, designed for use as an internal standard and tracer in analytical and environmental chemistry. Its superior isotopic purity and chemical stability make it indispensable for quantifying nitrosamine contaminants, validating analytical methods, and exploring reaction and degradation pathways. This compound plays a crucial role in modern toxicological research, environmental monitoring, and quality assurance for nitrosamine detection.